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mouse anti rpe 65  (Novus Biologicals)


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    Novus Biologicals mouse anti rpe 65
    Mouse Anti Rpe 65, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 95/100, based on 45 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+anti+rpe+65/RPE65+Antibody+(401%2E8B11%2E3D9)+-+BSA+Free/pmc11464875-236-4-6
    Average 95 stars, based on 45 article reviews
    mouse anti rpe 65 - by Bioz Stars, 2026-10
    95/100 stars

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    Related Articles

    other:

    Article Title: Annexin A2 Promotes Epiretinal Membrane Formation and Subsequent Proliferative Vitreoretinopathy in Response to a Macrophage Inflammatory Signal in Mice
    Article Snippet: Primary antibody combinations included mouse anti-RPE-65 (Novus NB100-355; 1:150) 324 with rabbit anti-annexin A2 (Cell Signaling 8235S; 1:300), rabbit anti-CD68 Abcam AB125212; 1:200) 325 with goat anti-annexin A2 (R&D Systems AF3928; 1:50), mouse anti-RPE-65 (Novus NB100-355; 1:150) 326 with rabbit anti-SMA (Cell Signaling 19245T; 1:300), and mouse anti-SMA (Cy3 Sigma C6198; 1:50) with 327 rabbit anti-CD68 (Abcam AB125212; 1:200).

    Article Title: Annexin A2 promotes proliferative vitreoretinopathy in response to a macrophage inflammatory signal in mice
    Article Snippet: Primary antibody combinations included mouse anti-RPE-65 (Novus NB100-355; 1:150) with rabbit anti-annexin A2 (Cell Signaling 8235S; 1:300), rabbit anti-CD68 (Abcam AB125212; 1:200) with goat anti-annexin A2 (R&D Systems AF3928; 1:50), mouse anti-RPE-65 (Novus NB100-355; 1:150) with rabbit anti-SMA (Cell Signaling 19245 T; 1:300), and mouse anti-SMA (Cy3 Sigma C6198; 1:50) with rabbit anti-CD68 (Abcam AB125212; 1:200).

    Article Title: Annexin A2 promotes proliferative vitreoretinopathy in response to a macrophage inflammatory signal in mice.
    Article Snippet: Primary antibody combinations included mouse anti-RPE-65 (Novus NB100-355; 1:150) with rabbit anti-annexin A2 (Cell Signaling 8235S; 1:300), rabbit anti-CD68 (Abcam AB125212; 1:200) with goat antiannexin A2 (R&D Systems AF3928; 1:50), mouse anti-RPE-65 (Novus NB100-355; 1:150) with rabbit anti-SMA (Cell Signaling 19245 T; 1:300), and mouse anti-SMA (Cy3 Sigma C6198; 1:50) with rabbit anti-CD68 (Abcam AB125212; 1:200).

    Incubation:

    Article Title: A Comprehensive Proteomic and Phosphoproteomic Analysis of Retinal Pigment Epithelium Reveals Multiple Pathway Alterations in Response to the Inflammatory Stimuli
    Article Snippet: After fixed with 4% paraformaldehyde for 15 min at room temperature, ARPE-19 cells were washed with PBS containing 0.3% Triton X-100 (PBST) three times and blocked at 37 °C for 1 h in PBST supplemented with 5% FBS (Gibco). .. Then, the cells were incubated with the following primary antibodies at 4 °C overnight: rabbit anti-ZO-1 (Santa Cruz Biotechnology Inc., Santa Cruz, CA, USA), mouse anti-Na + K + ATPase (Santa Cruz Biotechnology Inc.), and mouse anti-RPE-65 (Novus Biologicals, Littleton, CO, USA). .. Cells were washed 3 times for 5 min with PBST and incubated with Alexa Fluor 488-labeled goat anti-rabbit IgG (Molecular Probes, Eugene, OR, USA) and Alexa Fluor 635-labeled goat anti-mouse IgG (Molecular Probes) for 1 h at room temperature.



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    mouse anti-rpe-65 1：500  (Thermo Fisher)
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    Thermo Fisher mouse anti-rpe-65 1：500
    mRNA and protein expression of RPE markers. (A) The mRNA expression of <t>RPE-65,</t> BEST, CRLBP, MITF, PEDF, and ZO-1 was evaluated by quantitative real-time polymerase chain reaction. mRNAs were quantified from hES-RPE, ARPE-19, fetal RPE, and adult RPE normalized to the geometric mean of a housekeeping gene (18s rRNA). (B) The mRNA expression of pluripotency (OCT4 and NANOG), neuroectoderm (PAX6) and RPE markers (MITF, CRLBP, BEST, RPE-65, PEDF and ZO-1) was also compared between undifferentiated hESCs and hESC-RPE. As expected, compared to undifferentiated H1, differentiated cells at passage 2 presented lower expression of pluripotency genes, and higher expression of neuroectoderm and RPE differentiation markers. (C) Protein expression of BEST and MITF was evaluated in p2 hESC-RPE cells by immunofluorescence. As expected, BEST presented membrane localization, and MITF, nuclear localization. (D) Western Blotting analysis of RPE-65 and CRLBP expression by p1, p2 and p3 hESC-RPE cells. Similar to fetal RPE, hESC-RPE cells from all the analysed passages presented detectable expression of both RPE markers. Bars represent standard error of the mean. Abbreviations: hES-RPE, RPE derived from human embryonic stem cells; fRPE, fetal RPE.
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    mRNA and protein expression of RPE markers. (A) The mRNA expression of RPE-65, BEST, CRLBP, MITF, PEDF, and ZO-1 was evaluated by quantitative real-time polymerase chain reaction. mRNAs were quantified from hES-RPE, ARPE-19, fetal RPE, and adult RPE normalized to the geometric mean of a housekeeping gene (18s rRNA). (B) The mRNA expression of pluripotency (OCT4 and NANOG), neuroectoderm (PAX6) and RPE markers (MITF, CRLBP, BEST, RPE-65, PEDF and ZO-1) was also compared between undifferentiated hESCs and hESC-RPE. As expected, compared to undifferentiated H1, differentiated cells at passage 2 presented lower expression of pluripotency genes, and higher expression of neuroectoderm and RPE differentiation markers. (C) Protein expression of BEST and MITF was evaluated in p2 hESC-RPE cells by immunofluorescence. As expected, BEST presented membrane localization, and MITF, nuclear localization. (D) Western Blotting analysis of RPE-65 and CRLBP expression by p1, p2 and p3 hESC-RPE cells. Similar to fetal RPE, hESC-RPE cells from all the analysed passages presented detectable expression of both RPE markers. Bars represent standard error of the mean. Abbreviations: hES-RPE, RPE derived from human embryonic stem cells; fRPE, fetal RPE.

    Journal: International Journal of Stem Cells

    Article Title: Human Stem Cell-Derived Retinal Pigment Epithelial Cells as a Model for Drug Screening and Pre-Clinical Assays Compared to ARPE-19 Cell Line

    doi: 10.15283/ijsc20094

    Figure Lengend Snippet: mRNA and protein expression of RPE markers. (A) The mRNA expression of RPE-65, BEST, CRLBP, MITF, PEDF, and ZO-1 was evaluated by quantitative real-time polymerase chain reaction. mRNAs were quantified from hES-RPE, ARPE-19, fetal RPE, and adult RPE normalized to the geometric mean of a housekeeping gene (18s rRNA). (B) The mRNA expression of pluripotency (OCT4 and NANOG), neuroectoderm (PAX6) and RPE markers (MITF, CRLBP, BEST, RPE-65, PEDF and ZO-1) was also compared between undifferentiated hESCs and hESC-RPE. As expected, compared to undifferentiated H1, differentiated cells at passage 2 presented lower expression of pluripotency genes, and higher expression of neuroectoderm and RPE differentiation markers. (C) Protein expression of BEST and MITF was evaluated in p2 hESC-RPE cells by immunofluorescence. As expected, BEST presented membrane localization, and MITF, nuclear localization. (D) Western Blotting analysis of RPE-65 and CRLBP expression by p1, p2 and p3 hESC-RPE cells. Similar to fetal RPE, hESC-RPE cells from all the analysed passages presented detectable expression of both RPE markers. Bars represent standard error of the mean. Abbreviations: hES-RPE, RPE derived from human embryonic stem cells; fRPE, fetal RPE.

    Article Snippet: The membrane was washed with PBS-T (Tween-20 0.05%) and blocked with 5% BSA in PBS-T for 1 h. The membranes were incubated with mouse anti-RPE-65 1：500 (Pierce) and mouse anti-CRLBP 1：750 (Abcam) overnight at 4℃.

    Techniques: Expressing, Real-time Polymerase Chain Reaction, Immunofluorescence, Western Blot, Derivative Assay